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how to figure out the serum-ascites albumin gradient (SAAG), and why it matters so much.",{"id":215,"name":216},2,"Blog",[218,228,233,238,245],{"type":219,"data":220},"video",{"readDurationInSeconds":221,"video":222},194,{"platform":223,"name":212,"url":224,"size":225},"YouTube","https:\u002F\u002Fwww.youtube.com\u002Fwatch?v=xPOTWWCBsPQ",{"width":226,"height":227},200,113,{"type":229,"data":230},"free_text",{"readDurationInSeconds":231,"text":232},12,"\u003Cp>The cell count with differential is the single most useful test performed on ascitic fluid to evaluate for infection. 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You will understand the clinical implications of lab abnormalities and will know what to do about them.\u003C\u002Fp>",{"type":239,"data":240},"cta",{"readDurationInSeconds":241,"text":242,"buttonText":243,"buttonUrl":244},3.466666666666667,"Become a great clinician with our video courses and workshops","Start learning for free","https:\u002F\u002Fwww.medmastery.com\u002Fuser\u002Fregister",{"type":229,"data":246},{"readDurationInSeconds":247,"title":248,"text":249},83.2,"Video transcript","\u003Cp>In this Medmastery lesson, we will find out how the cell count and protein concentration helps us diagnose the cause of ascites. The cell count with differential is the single most useful test performed on ascitic fluid to evaluate for infection, and should be ordered on every ascites specimen, including therapeutic paracentesis specimen.\u003C\u002Fp>\u003Cp>Antibiotic treatment should be considered in any patient with a corrected neutrophil count equal to or greater than 250 per cubic millimeter. The presence of blood in a sample suggests a traumatic tap may have occurred. In this case, the white blood cells in the sample could come from two sources, the original ascitic fluid or the blood resulting from the trauma related to the procedure.\u003C\u002Fp>\u003Cp>The lab will perform a calculation to correct the white blood cell count in bloody samples. Remember that the protein concentration determines if the fluid is an exudate or transudate. Ascitic fluid is classified as an exudate if the total protein concentration is equal to or greater than 2.5 grams per deciliter, and a transudate if it is below 2.5 grams per deciliter.\u003C\u002Fp>\u003Cp>However, exudate transudate system of ascitic fluid classification has been replaced by the serum to ascites albumin gradient, which is a more useful measure for determining whether portal hypertension is present. The serum to ascites albumin gradient is easily calculated by taking the serum albumin value and subtracting the ascitic fluid albumin value.\u003C\u002Fp>\u003Cp>Both values should be obtained on the same day. The presence of a serum to ascites albumin gradient greater than or equal to 1.1 grams per deciliter, predicts that the patient has portal hypertension with 97 percent accuracy. 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